PDGF-BB에 의한 치주인대세포활성에 대한 TGF-β의 효과
The Effect of TGF-β1 on Cellular Activity of Periodontal Ligament Cells activated by PDGF-BB
소속 상세정보
백상철
박진우/서조영
KMID : 0363020020320030457
Abstract
The purposes of this study is to evaluate the combination effects of TGF-β1 and PDGF-BB on the periodontal ligament cells to use as a regeneration promoting agent of periodontal tissue. Human periodontal ligament cells were prepared from the first premolar tooth extracted for the orthodontic treatment and were cultured in DMEM/10% FBS at the 37℃, 5% CO2 incubator. Authors measured the DNA synthesis, total protein, collagen and noncollagenous protein synthesis according to the concentration of TGF-β1, (1, 5ng/ml) and PDGF-BB (1, 10 ng/ml) in combination. To explore further this delayed effect of TGF-β1, we preincubated human periodontal ligament cells with TGF-β1 for 4 or 24 hours before PDGF-BB stimulation.
The results were as follows:
The DNA synthetic activity was increased dose dependently by TGF-β1, PDGF-BB. The combination of TGF-β1 and PDGF-BB consistently enhanced the DNA synthetic activity to PDGF-BB alone. The ability of TGF-β1 to enhance DNA synthetic activity in PDGF-BB treated periodontal ligament cells was dose dependent. The maximum mitogenic effect was at the 5ng/ml of TGF-β1 and 10ng/ml of PDGF-BB. Preincubation of cells with TGF-β1 resulted in significantly greater response to PDGF-BB at all TGF-β1 concentration studied, and may be useful for clinical application in periodontal regenerative procedures. The total protein, collagen and noncollagen synthesis was increased dose pendently by TGF-β1, PDGF-BB. The % of collagen was slightly decresed according to the concentration of TGF-β1, PDGF-BB. The effect of TGF-β1, PDGF-BB were not specific for collagen synthesis since it also increased noncollagenous protein synthesis.
This study demonstrates that PDGF-BB is major mitogens for human periodontal ligament cells in vitro, and supports a role for TGF-β1 as a regulation of the mitogenic and total protein formation to PDGF-BB in these cells.
키워드
치주인대세포
PDGF-BB;TGF-β
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